page updated: 29.01.2009

ME » CURRICULUM VITAE ( 78 kb)



EDUCATION

2006 Computer Repair and Networking
2005 Advanced Course in High Resolution Molecular Cytogenetics
2004 St John’s Ambulance Senior First Aid Certificate
2004 Introduction & Advanced Microsoft Access
2002 Training in Laboratory Animal Care, Animal Use and Suture Methods
1999-2002 Bachelor of Science – Biotechnology (with Honours)
1997-1998 Higher School Certificate
1990-1997 Extracurricular Language Studies (Polish)

SCIENTIFIC EXPERIENCE

2008-[...] Research Assistant (on Secondment)
Centre for Infectious Diseases and Microbiology (CIDM), Westmead (Sydney)
Study of multiple resistance genes in bacteria of ICU patients.
2006-[...] Technical Officer
Institute of Clinical Pathology and Medical Research (ICPMR), Westmead (Sydney)
Molecular Pathology. Extraction of bacterial and viral DNA and RNA from many biological sources. Subsequent analysis of extracted nucleic acids using amplification techniques, followed by diagnosis, of negative results, or recommendation to registrar for diagnosis, for positive results.
2003-2005 Microarray Scientist
Hunter Area Pathology Service (HAPS), Newcastle
Production, implementation, and data analysis of microarrays requiring exceptional accuracy in data entry
2001-2003 Research Assistant & Industry Experience
University of Newcastle
Animal handling and dissection, data gathering and data entry
2002-2003 Demonstrator in Molecular Genetics
University of Newcastle
Requiring excellent commnuication skills targeted at students

ADDITIONAL EXPERIENCE

2002-2006 Casual Retail Assistant
Calendar Club, Charlestown Square
Customer service, stocktaking, cash handling
2005 Web Designer and Secretary
Web Dimensions, Central Coast
Web development using HTML, CSS and Macromedia Flash MX; and administrative including client service using telephone, email and in person
1996 Work Experience in Nursing
Obstetric and Surgical Units, Belmont District Hospital
1994 English Language Teacher
Poland
Teaching conversational English to school-aged children

AWARDS

2004 Ed Krumins Young Scientist Award with small scholarship
1997–1998 Unbroken attendance, years 11 and 12 (St Francis Xavier’s College)
1997 Distinction in The Rio Tinto Australian Science Olympiads, Biology National Qualifying Examination
1996 Dux in T.A.S. Technics (School Certificate)
1995 Merit for Academic Excellence in Science

PROFESSIONAL CONTRIBUTIONS

191 kb Peer-reviewed Publication
Aitken RJ, Bennetts LE, Sawyer D, Wiklendt AM, King BV.
Impact of radio frequency electromagnetic radiation on DNA integrity in the male germline.
Int J Androl. 2005; 28(3):171-9.
299 kb Peer-reviewed Publication
Sawyer DE, Mercer BG, Wiklendt AM, Aitken RJ.
Quantitative analysis of gene-specific DNA damage in human spermatozoa.
Mutat Res. 2003; 529(1-2):21-34.
  Oral Presentation
David Mowat, Kerry Fagan, Joan Dawes, Ian Dawes, Agnieszka Wiklendt, Bronwyn Robertson, Rohan Willams, Rodney Scott, Michael Buckley, Gillian Turner.
"THE "CHROMARRAY" GROUP".
DECIPHER Symposium held at the Wellcome Trust Genome Campus, Hinxton, England, 23-25 June 2005.
  Oral Presentation
Agnieszka Wiklendt, Katie Baines, Kerry Fagan, Gillian Turner, The ChromArray Group.
"Application of CGH Microarray Analysis for the Detection and Delineation of Unbalanced Chromosome Abnormalities".
10th Interim Meeting of Australasian Society of Cytogeneticists held in Hobart, Australia, March 2004.
  Poster Presentation
Lawrence O, Wiklendt A, Anderson J & NSW Chromarray Consortium.
"Production Of A 1 Mb Microarray For Analysis Of Chromosome Copy Number Changes By Array CGH".
Annual conference of the Human Genetics Society of Australasia, held in Newcastle, Australia, 25-29 July 2005.
Workshop brochure:
p1 254 kb
p2 253 kb
Workshop
Microarray Workshop: Participated as co-coordinator and presenter to 14 trans-Tasman participants including introduction, supervision, explanation and discussion of data analysis.
Held at the Hunter Area Pathology Service, Newcastle, 30th July 2005.
(brochure supplied as jpg images to reduce download size)
  Thesis
A. M. Wiklendt
"Effects of Radio Frequency Electromagnetic Radiation on Male Germ Cells of Mice (Mus musculus) in vivo."
Submitted 28 October 2002 as a part of the Bachelor of Science (Biotechnology) (Honours) Degree at the University of Newcastle, Australia.

SKILLS

Communication
  • Typing skills of 57 words per minute at 100% accuracy (certified July 2006)
  • Excellent language skills in English and Polish, and basic German
  • Co-Presenter and Co-coordinator of the 2005 HAPS Microarray Workshop
  • Gave successful oral presentation at the 44th Annual Australasian Society of Cytogenetics Conference held in Hobart, Tasmania 2004
  • Demonstrator of second year Molecular Biology labs, which included discussions with the class as a group, and individually with students
  • Successfully defended thesis and presentation in seminar conditions
  • Many dealings with clients, colleagues and sales representatives via telephone, conference calls, email, and VoIP (Voice over Internet Protocol) software
Computer
  • Fluorescent image capture programs: MetaSystems, Multianalyst, GenePix Pro
  • Microsoft Windows versions Vista, XP, 2000, NT, 97, 95, 3.11
  • Microsoft Office (Excel, Word, Access, PowerPoint) 2007, 2003, 2002, 2000, 95
  • Microsoft Works 2002, 95, 4.5a
  • Alternatives to MS Office, such as OpenOffice.org
  • Accurate data entry
  • Patient/Laboratory databases such as AUSLAB and PathNet, and various scientific and laboratory related software such as Pubmed, Medline, OVID, ensembl, GenePix Pro, Metqasystems, SigmaPlot, RASmol, and Multianalyst
  • Website Design including Notepad, Macromedia software, and FTP programs
  • DOS and DOS based programs
  • Imaging software such as Paintshop Pro, Adobe Photoshop, and GIMP
Molecular
  • DNA extraction from human, murine, bacterial and viral sources
    • of total nucleic acids from: sputums; aspirates; swabs; tissues (fresh/paraffin embedded); blood and blood products (including Guthrie cards); cultures; cerebrospinal, vitreous, amniotic, knee and other fluids; urines; bone marrows; and spermatozoa
    • using salt extraction, phenol/chloroform, boil or column methods
    • using robotic, centrifugation kits, and automated methods
  • DNA amplification and manipulation including
    • real-time multiplex-tandem PCR using a Corbett RotorGene machine
    • real-time duplex PCR using the SmartCycler system
    • real-time PCR using a Roche LightCycler machine
    • sub-typing of microbes using restriction enzymes on PCR products
    • sub-typing of microbes using ERIC PCR
    • determining directionality of an insert in a plasmid by PCR
    • manual quantitative PCR of large targets (up to 10.4 kilo bases)
    • degenerate oligo-primed (DOP) PCR and nick translation
    • optimisation of these techniques
  • Gel electrophoresis including
    • agarose and polyacrylamide gels using various buffers (TBE/TAE)
    • pulsed-field (bacterial subtyping, determining integrity of dsDNA)
    • alkaline (to determine ssDNA breaks)
    • neutral (to determine double-stranded and/or super-coiled DNA quality, quantity, and size)
    • documentation of images using digital and Polaroid systems
  • Reverse Line Blot including:
    • probe coupling to membrane
    • multiplex PCR of samples
    • hybridisation of samples to membrane
    • exposure of x-ray film and analysis of results
  • Fluorescent techniques, including
    • (the above mentioned real-time PCR applications)
    • labelling of DNA with in-house or various commercial probes
    • using DOP-PCR and Nick translation for labelling
    • Zeiss fluorescent microscopes with MetaSystems software
    • software for digital fluorescent image capture and enhancement
    • use of various fluorescent dyes including: Cy3, Cy5, A488, PicoGreen
    • for accurate DNA quantitation, in-situ hybridisation, protein detection
  • Microarray use, including
    • chip construction (custom in-house on Corning UltraGAPS slides)
    • chip preparation and hybridisation
    • scanning, data analysis and data interpretation
    • teaching and training (in workshops and for new team members)
Microbiological
  • Transformation of competent cells
  • Mating different species of bacteria on selective media
  • Streaking, spreading, patching and isolating bacteria using selective media
  • Streaking BAC/PAC cultures on plates to test for vitality and resistance
  • Storing BAC/PAC cultures on ceramic beads at -80°C
  • Preparation of broth and solid media with or without antibiotics
  • DNA extraction from bacterial preparations
  • Creating and maintaining a MS Access database to track both bacterial and DNA stocks
Cellular
  • Preparation of metaphase and interphase slides from blood cell cultures for cytogenetic analysis
  • Dark field, light field, fluorescent, confocal microscopy
  • Haemocytometer use and calculations
Animal Handling
  • Handling, care and observation
  • Administration of anaesthetics (subcutaneous, intra-muscular/venous)
  • Various methods of suturing
  • Dissection of male rodent reproductive organs and isolation of spermatozoa
    • Using cannulation by specialised (safe!) mouth pipette
    • Using perforation and forced extraction under BWW in mineral oil
Technical and Other laboratory skills
  • Preparation of broth and agar media
  • Bioinformatics
  • Report and document filing and retrieval
  • Producing and updating documentation (Standard Operation Procedures (SOPs), Material Safety Data Sheets (MSDSs))
  • Reagent mixing, gel making, compliance with OH&S for chemical labelling, handling, and disposal (including handling of hazardous substances)
  • Glassware and equipment care, cleaning, and maintenance
  • Proper use of laboratory equipment, consumables and reagents
  • Version control and backing up of computer generated files

LEISURE ACTIVITIES and INTERESTS

Sporting Kayaking, squash, cycling, walking, softball, kickboxing, horse riding
Reading Quantum physics, science biographies, numeracy, human psychological development, thriller, sci-fi, fantasy, poetry
Other Web design, database design, Japanese culture; cooking; gardening

REFERENCES

references are withheld online. please contact me for enquiries. phoenix.webber@gmail.com